PROTEIN IMPORT INTO PEROXISOMES
Prof. Dr. Ralf Erdmann
since 2009: Chair of System Biochemistry (W3), Faculty of Medicine, RUB
2002-2009: Chair of System Biochemistry (C4), Faculty of Medicine, RUB
1998-2002: Associate Professor (C3) of Biochemistry, Free University Berlin
1995-1998: Assistant Professor (C1), RUB
1994-1995: Research Associate, Howard Hughes Medical Institute, New York
1991-1994: Rockefeller University, Lab of Günter Blobel, New York
1989-1991: Research Associate, RUB
1986-1989: PhD, Ruhr- University Bochum (RUB)
Peroxisomes are ubiquitous cellular organelles that are surrounded by a single membrane, multiply by division and de novo formation from the ER and play essential roles in lipid metabolism. Defects in peroxisome function of biogenesis are responsible for devastating human disorders, the mostly lethal peroxisomal diseases. Peroxisomal matrix proteins contain specific peroxisomal targeting signals (PTS1 or PTS2) that are post-translationally recognized and bound in the cytosol by the peroxisomal import receptors, which direct the receptor-cargo complex to the peroxisomal membrane. The cargo-loaded receptors insert into the peroxisomal membrane and assemble with other membrane proteins to form the translocon, which as a transient pore allows the translocation of the folded proteins across the membrane.
• Biogenesis and function of peroxisomes
• Structure and dynamics of the peroxisomal translocon
• Elucidation of the mechanism of the translocation of folded protein across the peroxisomal membrane
• Regulation of the function of the translocon
All common biochemical, cell biological and molecular biological techniques are established in the lab.
Neuhaus A, Kooshapur H, Wolf J, Meyer NH, Madl T, Saidowsky J, Hambruch E, Lazam A, Jung M, Sattler M, Schliebs W, Erdmann R. A Novel Pex14 Protein-interacting Site of Human Pex5 Is Critical for Matrix Protein Import into Peroxisomes. J Biol Chem 2014, 289, 437-48.
Hasan S, Platta HW, Erdmann R. Import of proteins into the peroxisomal matrix. Front Physiol 2013, 4, 261.
Meinecke M, Cizmowski C, Schliebs W, Krüger V, Beck S, Wagner R, Erdmann R. The peroxisomal importomer constitutes a large and highly dynamic pore. Nat Cell Biol 2010, 12, 273-7.
Schliebs W, Girzalsky W, Erdmann R. Peroxisomal protein import and ERAD: variations on a common theme. Nat Rev Mol Cell Biol 2010, 11, 885-90.
Platta HW, El Magraoui F, Schlee D, Grunau S, Girzalsky W, Erdmann R. Ubiquitination of the peroxisomal import receptor Pex5p is required for its recycling. J Cell Biol 2007, 177, 197-204.
Platta HW, Grunau S, Rosenkranz K, Girzalsky W, Erdmann R. Functional role of the AAA peroxins in dislocation of the cycling PTS1 receptor back to the cytosol. Nat Cell Biol 2005, 8, 817-822.
Erdmann and Schliebs W. Peroxisomal matrix protein import: the transient pore model. Nat Rev Mol Cell Biol 2005, 6, 738-42.