wu yaowen-webMEMBRANE TRAFFICKING AND AUTOPHAGY

Dr. Yaowen Wu

2012-present: Group Leader, Chemical Genomics Centre of the Max Planck Society
2010-present: Otto-Hahn Group Leader, MPI of Molecular Physiology
2009-2010: Postdoc in Cell Biology, King’s College London
2008: Ph.D. (summa cum laude), MPI of Molecular Physiology (examination at the TU Dortmund)
2004: Msc. in Organic Chemistry, Tsinghua University

Research Interest
My laboratory is interested in understanding the regulation mechanism and membrane morphogenesis of autophagy (a self-eating process in eukaryotes) and membrane trafficking and signal transduction regulated by small G-proteins. Research projects are of biomedical relevance and pursue methodological advancement. Our work lies at the interface between chemistry and biomedicine. On one hand, we develop novel chemical and synthetic approaches that open up new avenue for manipulating protein function and visualizing biological processes in live cells. On the other hand, the application of the diverse tools shed light on the mechanism of autophagy and membrane trafficking.


Techniques
Biochemistry and molecular biology; protein/DNA engineering; biosensor; chemical and optogenetics; chemical synthesis; chemical protein synthesis and labeling; imaging of cellular events; cell biology; synthetic biology.

Selected Reading
Zhao L, Ehrt C, Koch O, Wu YW. One-Pot N2C/C2C/N2N Ligation to Trap Weak Protein-Protein Interactions. Angew Chem Int Ed 2016, doi: 10.1002/anie.201601299.

Voss S, Klewer L, Wu YW. Chemically induced dimerization: reversible and spatiotemporal control of protein function in cells. Curr Opin Chem Biol 2015, 28: 194–201.

Yang A, Zhao L, Wu YW. Chemical synthesis and biological function of lipidated proteins. Topics Curr Chem 2015, 362: 137-82.

Liu P, Calderon A, Hou J, Konstantinidis G, Chen X, Fu L, Banerjee S, Dehmelt L, Wu YW. A bioorthogonal small-molecule switch system for controlling protein function in live cells. Angew Chem Int Ed  2014, 53 (38):10049-55.


Liu W, Li F, Chen X, Yi L, Wu YW. A rapid and fluorogenic TMP-AcBOPDIPY probe for covalent labeling of proteins in live cells. J Am Chem Soc 2014, 136 (12): 4468-71.

Li F, Yi L, Zhao L, Itzen A, Goody RS, Wu YW. The role of the hypervariable C-terminal domain in Rab GTPases membrane targeting. Proc Natl Acad Sci USA 2014, 111(7), 2572-7.

Deraeve C, Guo Z, Bon RS, Blankenfeldt W, DiLucrezia R, Wolf A, Menninger S, Stigter EA, Wetzel S, Choidas A, Alexandrov K, Waldmann H, Goody RS, Wu YW. Psoromic acid is a selective and covalent Rab-prenylation inhibitor targeting autoinhibited RabGGTase. J Am Chem Soc 2012, 134 (17), 7384-91.

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