MOLECULAR CELL BIOLOGY
Prof. Dr. Konstanze F. Winklhofer
Current Position: Chair, Molecular Cell Biology, Institute of Biochemistry and Pathobiochemistry, Ruhr University Bochum
Group Leader: Ludwig Maximilians University Munich, Neurobiochemistry
Dr. rer. nat. habil (Ph.D.): Max-Planck-Institute for Biochemistry, Department of Cellular Biochemistry (Prof. Dr. F. U. Hartl)
Dr. med. (M.D.): Max-Planck-Institute for Biochemistry, Molecular Virology
Studies: Pharmaceutical Sciences (University Regensburg) and Medicine (Ludwig Maximilias University Munich)
We are interested in cellular quality control mechanisms that maintain neuronal integrity and are impaired in neurodegenerative diseases.
Neuroprotection and ubiquitin signaling
We are studying how different modes of ubiquitination influence prosurvival signaling pathways, which E3 ubiquitin ligase are involved and how they are regulated.
Mitochondria and stress protection
Another focus of our work is the role of mitochondria as key organelles in orchestrating cell death and viability and in regulating neuronal bioenergetics. In this context we are are studying the role of interorganellar crosstalk in stress response pathways.
Cell biology, molecular biology and biochemistry:
Cell death and viability assays, reporter gene assays, ubiquitination assays, immunocytochemitry, immunoblotting, immunoprecipitation;
preparation of primary neurons;
confocal laser scanning microscopy
Winklhofer KF. Parkin and mitochondrial quality control: toward assembling the puzzle. Trend Cell Biol 2014, 24(6), 332-41.
Müller-Rischart AK, Pilsl A, Beaudette P, Patra M, Hadian K, Funke M, Peis R, Deinlein A, Schweimer C, Kuhn PH, Lichtenthaler SF, Motori E, Hrelia S, Wurst W, Trümbach D, Langer T, Krappmann D, Dittmar G, Tatzelt J, Winklhofer KF. The E3 ligase parkin maintains mitochondrial integrity by increasing linear ubiquitination of NEMO. Mol Cell 2013, 49(5), 908-21.
Rolland SG, Motori E, Memar N, Hench J, Frank S, Winklhofer KF, Conradt B. Impaired complex IV activity in response to loss of LRPPRC function can be compensated by mitochondrial hyperfusion. Proc Natl Acad Sci USA 2013,110(32), E2967-76.
Bouman L, Schlierf A, Lutz AK, Shan J, Deinlein A, Kast J, Galehdar Z, Palmisano V, Patenge N, Berg D, Gasser T, Augustin R, Trümbach D, Irrcher I, Park DS, Wurst W, Kilberg MS, Tatzelt J, Winklhofer KF. Parkin is transcriptionally regulated by ATF4: evidence for an interconnection between mitochondrial stress and ER stress. Cell Death Differ 2011, 18, 769-782.